Cladosporioides, and established a four-seat nested multiplex PCR assay. Alternata was found to be 10 fg/μL, and 10 pg/μL for C. In the actual application test, our established nested multiplex PCR can detect pathogens more quickly and accurately than conventional culture method. This method combines the advantages of nested and multiplex techniques, and it considerably improves the sensitivity and specificity of the detection results. Therefore, we hope that this method can provide a tool for diagnosing D.
Use our online Applied Biosystems™ Primer Designer™ Tool to search for the right PCR/Sanger sequencing primer pair from a database of ~650,000 predesigned primer pairs for resequencing the human exome and human mitochondrial genome. Tibeticum samples (five individuals from each population) for amplification efficiency and polymorphism.From design to synthesis, quality primers are vital to successful results. Streamline your workflow with our primer design toolNinety-four primer pairs were designed for microsatellite loci candidates using Primer Premier 5.0 software (Premier Biosoft International, Palo Alto, California, USA) and tested with 15 C. With the automatic search, the program gradually relaxes the stringency of the search until the best possible primers available within. Primers can be designed automatically or manually. PRIMER PREMIER PRO is one of the most comprehensive primer design software available to design optimal primers for PCR, sequencing and hybridization quickly.
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Full primer coverage for Ion Torrent™ AmpliSeq™ Exome Panel and Ion Torrent™ AmpliSeq™ Cancer Hotspot Panel v. Our primers are free of known SNPs and primer-dimers, highly target-specific and used under universal PCR conditions
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